Lab Systems

Heska

“We’re a long-time Heska customer. When I purchased my second clinic, I chose to bring Heska with me and replaced their brand new ProCyte from Idexx, with Heska’s HemaTrue. I don’t know why anyone would use anything else. The cost per test is unbeatable and it’s so easy to use.”

- Matt Dyer, Practice Owner
Wixom Family Pet Practice

HemaTrue Hematology Analyzer

The HemaTrue analyzer delivers accurate results in only 55 seconds and with only 20 µL (about one drop) of blood. Ease-of-use features include a color LCD touch-screen, step-by-step instruction screens, an on-board blood mixer, and an integrated bar code reader.
Watch a video.

EASY TO USE

Hema True Hematology Analyzer
  • Color LCD Touch-Screen: Provides easy navigation.
  • Step-by-Step Instruction Screens: System prompts reduce the need for training.
  • On-board Blood Tube Mixer: Mix samples in as little as one minute.
  • 2 ways to Introduce a Sample:
    1. Whole Blood Sample Probe: Aspirates from an EDTA tube.
    2. True20™ Sampling for Small or Dehydrated Patients: Requires only 20 μl (about one drop) of blood.
  • Integrated Bar Code Reader: Managing quality control is faster and easier than ever.
  • Color-Coded Reagent System: A convenient tray houses reagent bottles; color-coded caps protect reagents from environmental contaminants.

 
The HemaTrue® Veterinary Hematology Analyzer delivers excellent precision and accuracy across a wide range of values.

Precision (Reproducibility)

Precision, or reproducibility, is a statistical measurement of how well an analyzer can “reproduce” the same value if a single sample is run multiple times on the same instrument. Precision is your assurance that an analyzer is producing consistent, reliable results. A high-quality analyzer should be able to generate values that are fairly close to one another across all sample runs.

Heska's precise and on target results.

Correlation (Accuracy)

Correlation (or accuracy), can only be achieved after precision is assured. Accuracy is determined by running numerous samples on a “test” analyzer then running the same samples on a reference instrument. A statistical correlation, known as the r value, reflects how similar results are between the two instruments. In a recent study, 367 dog, cat, and horse samples were run on the HemaTrue® analyzer and also on a reference instrument—the Bayer ADVIA® 120 system. Close alignment of the HemaTrue analyzer results to the red “perfection” line shows that the HemaTrue analyzer results are virtually indistinguishable from the comparable reference lab results.

Precise results comparable to reference lab results

The HemaTrue Systems results are proven to be nearly perfect compared to reference lab tests

SOUND SCIENCE

Impedance Technology
Impedance technology is a proven, well-established method for deriving many components of the complete blood count (CBC).

While it may seem counter-intuitive that a 3-part differential would provide more reliable diagnoses than a 5-part differential, numerous studies have demonstrated that:
  • Impedance technology produces a granulocyte measurement that is highly consistent from animal to animal.
  • The behavior of eosinophils is inconsistent. In some animals, eosinophils may merge with neutrophils or monocytes, even on the Bayer ADVIA® 120 system (gold standard).
  • No veterinary hematology system has been validated for the ability to enumerate basophils.
  • The inconsistent behavior of animal WBC in light scatter systems and the high incidence of undetected pathologic abnormalities by all instrument systems are such that most laboratories perform a microscopy differential on all samples.
  • Some abnormalities may occur in the WBC that cannot be identified by any instrument system and are only detected by scanning stained blood films.
  • Software configurations associated with impedance technology are more adaptable to a wider range of species than light scatter systems.
The HemaTrue analyzer is proven to consistently and reliably identify abnormalities that require blood film review. Using blood films in conjunction with a hematology system, the practitioner can verify there are no abnormalities in the instrument-generated differential. Abnormalities beyond the expertise of the in-hospital laboratory may then be referred to a clinical pathologist for review.

Precise Sample Measurement and Cell Counting
The HemaTrue analyzer uses technologies found in high-end reference analyzers to ensure precise sample measurement. Utilizing shear valve technology, it “shears off” a very precise amount of sample for dilution. Since counts are reported as X number of cells per microliter of blood, the quantity of blood used for analysis must be precise and reproducible. The shear valve excels at this task for making dilutions.

The HemaTrue analyzer also utilizes metering tubes to accurately count the number of cells in a given volume of diluted blood. Other analyzers count cells by drawing sample through the aperture for a fixed period of time, for example, 10 seconds. This may lead to inaccurate cell counts if the opening is restricted due to build-up of protein from previous blood samples. Fewer cells pass through the orifice during the 10-second interval. Metering tube technology allows the HemaTrue analyzer to count cells based on a fixed volume, not a fixed time. If the aperture opening is restricted, the sample flows slower through the aperture but also flows slower through the metering tubes. Regardless of the speed, cell counting is initiated at the “Start” point and ends at the “Stop” point. The volume of fluid in the metering tube (270 μl), and thus the volume of sample drawn through the aperture, remains the same time after time.

Exclusive True20™ Sampling
The HemaTrue analyzer gives you the ability to run a complete CBC with a 20-μl sample (about one drop)—a significant benefit with small or dehydrated patients. True20™ Sampling involves the use of a 20-μl EDTA-coated capillary tube and an adapter for use in the analyzer. To use True20™ Sampling:

Instructions on True20 Sampling
  1. Place a needle into an accessible vein (do not attach a syringe!).
  2. Allow blood to fill the hub of the needle.
  3. Place one end of the True20™ capillary tube into the needle hub and allow the tube to fill.
  4. Wipe the outside of the capillary tube to remove excess blood.
  5. Place the tube into the Micropipette Adapter.
  6. Place the Micropipette Adapter into the HemaTrue analyzer. Results are generated in less than one minute.

ADVANCED DIAGNOSTICS

Cell Distribution
The HemaTrue analyzer plots results on a size distribution graph called a histogram. Small cells are plotted on the left side of the histogram; large cells are plotted on the right. Typical histograms appear as follows:

RBC/PLT Histogram & WBC Differential Histogram

All hematology analyzers use size as a criterion for cell differentiation. Smaller cells, such as platelets, are “seen” differently than larger cells, such as RBCs. Cell size varies considerably from species to species and patient to patient.

Floating Discriminators
Floating DiscriminatorsMost in-house hematology systems use fixed separation lines, or discriminators, to distinguish populations. These lines are fixed in place on the histogram, based on where cell populations typically fall. However, this technique can be inaccurate if cells are smaller or larger than “average.”

The HemaTrue analyzer utilizes floating rather than fixed discriminators to differentiate cell types. With each patient, an advanced software program evaluates the histograms and calculates the optimal way to separate out each cell type. This produces a very accurate count of each population. The divisions between cell types will vary from patient to patient based on species and disease condition. Because of this fluctuation, discriminators that are fixed in place will often give erroneous results. Floating discriminators, on the other hand, are placed at optimum separation points based on individual patient data.

WBC Pathology
The HemaTrue analyzer plots a WBC differential using a technique similar to RBC/PLT analysis. In the WBC dilution, a lysing reagent is added, which removes RBCs and platelets and strips membranes from WBCs. This leaves nuclear remnants, which are drawn through the WBC aperture then counted and sized just as though they were intact cells. A WBC histogram is then generated, showing smallest to largest-sized remnants. Lymphocytes form the smallest remnant and are plotted to the far left. Monocytes fall in the middle of the histogram, and granulocytes (neutrophils, eosinophils, and basophils) fall to the far right. The height of each histogram represents the number of cells of each size. Histograms are used in conjunction with cell counts and instrument flags to identify abnormalities in the CBC. Any abnormality should then be followed up by examination of a blood film.

Normal histogram and Leukemic condition histogram

Reticulocytes
Anemia is typically present in 5–10% of small animal samples. In these instances, it is useful to evaluate the blood for the presence of regeneration and RBC abnormalities that may help determine the cause of the anemia. Heska recommends that the best way to assess regeneration is examination of the stained blood film for polychromasia (or reticulocytes), while also looking for RBC abnormalities. The HemaTrue analyzer’s identification of an increase in RDW and/or MCV is evidence of probable reticulocytosis, but this is best confirmed by slide examination.

Intelligent Information Messages The HemaTrue system provides intelligent information messages related to pathology present in the sample. These messages are intended to be a guide to sample result criteria for slide review that may be used in a laboratory. We anticipate you may see these messages in up to 50% of the samples you analyze. This is not an indication of a system problem. It is an indication that additional diagnostic information may be found on the slide to supplement the instrument results. Examples of pathology messages from the HemaTrue analyzer include:
  • Message: Leukopenia; recommend scanning slide: The total WBC and differential are reliable. Pathology of WBC often associated with leukopenia may be detected on slide review. Review blood film for presence of neutrophil toxic change and/or left shift. This is useful to differentiate severe inflammatory consumption from bone marrow injury.
  • Message: Anemia; evaluate RBC on slide: When significant anemia is present, it is typical to evaluate RBC for clues to the cause of anemia.
  • Message: Evaluate platelets on slide: When platelets are decreased below certain level, it is advised to rule out platelet clumping on slide as cause. Certain differential results will also suggest that the slide be reviewed by scanning to make sure that the slide does not contain abnormal WBC that may not be detectable by an instrument system.

TEST MENU

PARAMETERS:

Total White Blood Cell Count (WBC)

Lymphocyte concentration in absolute number and percentage (LYMPH)

Monocyte concentration in absolute number and percentage (MONO)

Granulocyte concentration in absolute number and percentage (GRAN)

WBC size distribution histogram Hematocrit (HCT)

Mean Cell Volume of RBCs (MCV)

Red Cell Distribution Width in absolute number and percentage (RDW)

RBC size distribution histogram Hemoglobin Concentration (HGB)

Mean Cell Hemoglobin Concentration (MCHC)

Mean Cell Hemoglobin (MCH)

Total Red Blood Cell Count (RBC)

Total Platelet Count (PLT)

Mean Platelet Volume (MPV)

PLT size distribution histogram

SPECIFICATIONS

Specifications Description
Measuring Principle RBC, WBC, PLT Impedance, with individual cell analysis
Measuring Principle HGB Photometer, Cyanide-free method 535 nm ±5 nm
Discriminator Configurations Pre-programmed for dog, cat, rabbit, ferret, horse and cow. Additional discriminator configurations available upon request.
Sampling System Closed shear valve
Parameters Reported Leukocytes: WBC, LYMPH#, MONO#, GRAN#, LYMPH%, MONO%, GRAN%, WBC Histogram Erythrocytes: HCT, MCV, RDWa, RDW%, HGB, MCHC, MCH, RBC, RBC Histogram Platelets: PLT, MPV, PLT Histogram
Aspirated Blood Volume (open tubes) <125 μl
Blood Volume Using the Micropipette Adapter (MPA) 20 μl
LCD Graphical color touch screen, 240 columns x 320 rows
Keyboard Virtual incorporated keyboard (external keyboard optional)
Analysis Time <1 minute
QC Capabilities Complete automation, including positive lot and expiry monitoring, result evaluation, and trend-tracking with Levy-Jennings plots.
Floating Discriminator WBC/PLT Yes (position printed)
Automatic HGB Blank on Each Sample Yes
Bar Code Reader Input Yes
Temperature 18–32° C (64–90° F)
Humidity (none condensing) Up to 80%
Analyzer Dimensions (H x W x D) 16.1 in. x 11.4 in. x 18.1 in.
Weight 39.68 lbs.
Data Transfer RS–232C interface (1 port), USB interface (3 ports)