Using a refractometer, determine the specific gravity of the urine sample.
Prepare and dispense urine into the E.R.D.-HealthScreen® Urine Test Sample Dilution Tube
according to the following instructions:
If the specific gravity is less than 1.020
Add the urine sample to the line marked 1.020
If the specific gravity is 1.020 or above (to the upper limit of the refractometer)
Add the urine sample (1 mL) to the line marked "Sample"
Add distilled water to the line that matches the specific gravity of the
urine sample
If the specific gravity is greater than the upper limit of the refractometer
Dilute the urine sample 50:50 — use 1 mL of urine and 1 mL of distilled water
— in a separate container (i.e., not the Sample Dilution Tube)
Measure the specific gravity of the diluted sample — this is the specific gravity
utilized for running the test
Add the diluted sample (1 mL) to the line marked "Sample"
Add distilled water to the line that matches the specific gravity of the diluted sample
NOTE: Following the instructions in Step 2 allows each urine sample to be normalized (i.e., standardized) prior to performing the test. Therefore, specific gravity variations will not affect comparison of sequential urine samples from the same dog or cat.
Cap the Sample Dilution Tube and mix the sample by inverting the tube 3 times.
Remove the Test Device from the foil pouch and place it into the Sample Dilution Tube.
Move the Test Device up and down twice to ensure proper sample flow.
Leave the Test Device in the urine sample for a minimum of 3 minutes (not to exceed 1 hour).
Remove the Test Device and read results by comparing relative intensities of the colored
band(s) in the test window (See "Reading a Test Result").
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