HemaTrue^®

Best-in-Class Impedance Technology

Impedance technology is a proven, well-established method for deriving many components of the complete blood count (CBC).

While it may seem counter-intuitive that a 3-part differential would provide more reliable diagnoses than a 5-part differential, numerous studies have demonstrated that:

• Impedance technology produces a granulocyte measurement that is highly consistent from animal to animal.
• The behavior of eosinophils is inconsistent. In some animals, eosinophils may merge with neutrophils or monocytes, even on the Bayer ADVIA® 120 system (gold standard).
• No veterinary hematology system has been validated for the ability to enumerate basophils.
• The inconsistent behavior of animal WBC in light scatter systems and the high incidence of undetected pathologic abnormalities by all instrument systems are such that most laboratories perform a microscopy differential on all samples.
• Some abnormalities may occur in the WBC that cannot be identified by any instrument system and are only detected by scanning stained blood films.
• Software configurations associated with impedance technology are more adaptable to a wider range of species than light scatter systems.

The HemaTrue analyzer is proven to consistently and reliably identify abnormalities that require blood film review. Using blood films in conjunction with a hematology system, the practitioner can verify there are no abnormalities in the instrument-generated differential. Abnormalities beyond the expertise of the in-hospital laboratory may then be referred to a clinical pathologist for review.

Precise Sample Measurement and Cell Counting

The HemaTrue analyzer uses technologies found in high-end reference analyzers to ensure precise sample measurement. Utilizing shear valve technology, it “shears off” a very precise amount of sample for dilution. Since counts are reported as X number of cells per microliter of blood, the quantity of blood used for analysis must be precise and reproducible. The shear valve excels at this task for making dilutions.

The HemaTrue analyzer also utilizes metering tubes to accurately count the number of cells in a given volume of diluted blood. Other analyzers count cells by drawing sample through the aperture for a fixed period of time, for example, 10 seconds. This may lead to inaccurate cell counts if the opening is restricted due to build-up of protein from previous blood samples. Fewer cells pass through the orifice during the 10-second interval. Metering tube technology allows the HemaTrue analyzer to count cells based on a fixed volume, not a fixed time. If the aperture opening is restricted, the sample flows slower through the aperture but also flows slower through the metering tubes. Regardless of the speed, cell counting is initiated at the “Start” point and ends at the “Stop” point. The volume of fluid in the metering tube (270 μl), and thus the volume of sample drawn through the aperture, remains the same time after time.

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