Element HT5 - Veterinary Hematology Analyzer
The new Element HT5® provides a true 5-part WBC differential, RBC and PLT Parameters using laser, impedance and colorimetric technologies. With just 15uL of sample, get results in under 1 minute with proven accuracy.  Watch a Video

EASY TO USE Photo of Element HT5 Analyzer
  • Results within 60 seconds including a true 5-part White Blood Cell differential, Red Blood Cell and Platelet parameters, from a 15 μL sample.

  • Available Species: Dog, Cat, Horse, Cow, Ferret, Goat, Llama, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Giant Panda, and Red Panda.

  • Large, color touch screen with intuitive navigation simplifies patient data entry and viewing results.

  • View numeric results, histograms and scatter plots.

  • Simple reagent management via bar-code scanner and on-board menu functions. Internal reagent storage saves space and reduces clutter.

How accurate is the new Element HT5 veterinary hematology analyzer from Heska?

To define accuracy, we need to look at 2 metrics: Reproducibility and Correlation.

Reproducibility is a statistical measurement of how well an analyzer can “reproduce” the same value if a single sample is run multiple times on the same instrument. Precision is your assurance that an analyzer is producing consistent, reliable results. A high-quality analyzer should be able to generate values that are fairly close to one another across all sample runs.

Without excellent reproducibility, it is not possible to achieve accuracy!

If a system has solid demonstrated reproducibility, is calibrated to a standard, and calibration is monitored by a valid quality control (QC) program, then the user can be assured of analytical reliability.

Below are plots of representative reproducibility of 20 sequential replicate analysis of dog blood run on the Element HT5: (each plot with label: Typical performance range of QC program at this/these values is/are indicated by bracketed range)
  1. WBC plot
  2. MCV/HCT/HGB/RBC plot
  3. PLT plot
WBC plot MCV/HCT/HGB/RBC Plot PLT Plot
Summary Points:
  • Good reproducibility is expected to be tighter than the tolerance limits of a QC program.
  • Element HT5 reproducibility is excellent
Correlation is determined by running numerous samples on a “test” analyzer then running the same samples on a reference instrument. A statistical correlation, known as the r value, reflects how similar results are between the two instruments. The Element HT5 was tested in a major veterinary teaching hospital setting using established procedures for the analysis of dog, cat and horse blood from animals with a wide variety of medical conditions. Samples from these animals represented a wide variety of hematologic abnormalities. These same samples were also run on a reference laboratory analyzer – the Bayer ADVIA® 120 Hematology System and differential results performed by 100 cell microscopy counts.

Data presented here include analysis of blood from 97 dogs, 60 cats, and 49 horses. Scatterplots consist of >200 points and therefore contain superimposed points. Total WBC Plot RBC Plot HCT Plot PLT Plot
Summary Points:
  • There is excellent correlation for a population of animals measured on two different technologies.
  • Dynamic platelet clumping contributes to scatter in all platelet correlation studies.
The Element HT5 Hematology Analyzer offers:
  • A True 5-part White Blood Cell differential, Red Blood Cell and Platelet parameters
  • Results in less than a minute from a 15uL sample
  • Pre-Dilute Mode
Measurement methods used are:
  • Electrical Impedance method for determining the RBC and PLT data
  • Colorimetric Method for determining the HGB
  • Flow Cytometry by Laser for determining the WBC data.
Other parameter results are obtained via calculation.

Combination of Technologies: DIFF Channel, RBC/PLT Channel, Baso/HGB Channel

Electrical Impedance Method:
RBCs/PLTs are counted and sized by the Electrical Impedance method. This method is based on the measurement of changes in electrical resistance produced by a particle, which in this case is a blood cell, suspended in a conductive diluent as it passes through an aperture of known dimensions. A pair of electrodes is submerged in the liquid on both sides of the aperture to create an electrical pathway. As each particle passes through the aperture, a transitory change in the resistance between the electrodes is produced. This change produces a measurable electrical pulse. The number of pulses generated represents the number of particles that passed through the aperture. The amplitude of each pulse is proportional to the volume of each particle.

Output includes numeric values, percentages plus histograms for RBC, WBC & PLT plus scatter plots for analyzing cell volume, complexity and granularity. Images of each from the sales sheet

Flow Cytometry by Laser Flow Cytometry by Laser
After a predetermined volume of blood is aspirated and diluted by a certain amount of reagent, it is injected into the flow cell. Surrounded with sheath fluid (diluent), the blood cells pass through the center of the flow cell in a single column at a faster speed. When the blood cells suspended in the diluent pass through the flow cell, they are exposed to a laser beam.

The intensity of scatter light reflects the blood cell size and intracellular density. The low-angle scattered light reflects cell size, and the high-angle scattered light reflects intracellular density (nucleus size and density). The optical detector receives this scatter light and converts it into electrical pulses. Pulse data collected can be used to draw a 3-dimensional distribution (scattergram).

Low Angle Scatter, Mid Angle Scatter, Wide Angle ScatterTriple angle scatter detects cell volume, complexity and granularity of cells.


Multiple angle scatterplots aid to differentiate WBC differential.

Low Angle Scatter (LAS)-Detects Cell Volume
Mid Angle Scatter (MAS)- Detects Cellular Complexity
Wide Angle Scatter (WAS)-Detects Cellular Granularity

Multiple angle scatterplots aid to differentiate WBC differential. Multiple Angle Scatterplots

Colorimetric Method
The WBC/HGB dilution is delivered to the HGB bath where it is bubble mixed with a certain amount of lyse, which converts hemoglobin to a hemoglobin complex that is measurable at 530 nm. An LED is mounted on one side of the bath and emits a beam of monochromatic light, whose central wavelength is 530 nm. The light passes through the sample and is then measured by an optical sensor that is mounted on the opposite side. The signal is then amplified and the voltage is measured and compared to the blank reference reading (readings taken when there is only diluent in the bath), and the HGB is measured and calculated in the analyzer automatically.

Colometric Method

Reticulocytes
Anemia is typically present in 5–10% of small animal samples. In these instances, it is useful to evaluate the blood for the presence of regeneration and RBC abnormalities that may help determine the cause of the anemia. Heska recommends that the best way to assess regeneration is examination of the stained blood film for polychromasia (or reticulocytes), while also looking for RBC abnormalities. The Element HT5 analyzer’s identification of an increase in RDW and/or MCV is evidence of probable reticulocytosis, but this is best confirmed by slide examination.

Sample Pathology Messages
The Element HT5 system provides intelligent information messages related to pathology present in the sample. These messages are intended to be a guide to sample result criteria for slide review that may be used in a laboratory. These messages are indications that additional diagnostic information may be found on the slide to supplement the instrument results.

20 TEST PARAMETERS

White Blood Cell Red Blood Cell
Total White Blood Cell (WBC) Total Red Blood Cell (RBC)
Neutrophil Number (NEU#) Hemoglobin (HGB)
Neutrophil Percent (NEU%) Hematocrit (HCT)
Lymphocyte Number (LYM#) Mean Cell Volume (MCV)
Lymphocyte Percent (LYM%) Mean Cell Hemoglobin (MCH)
Monocyte Number (MON#) Mean Cell Hemoglobin Conc. (MCHC)
Monocyte Percent (MON%) Red Blood Cell Distribution (RDW-CV)
Eosinophil Number (EOS#)
Eosinophil Percent (EOS%) Platelet
Basophil Number (BAS#) Total Platelet (PLT)
Basophil Percent (BAS%) Mean Platelet Volume (MPV)

Available Species:

Dog, Cat, Horse, Cow, Ferret, Goat, Llama, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Giant Panda, and Red Panda.

Interfaces:

4 USB ports
1 Ethernet Port

Power Supply:

Voltage Input power Frequency: Analyzer (100V-240V~)±10% 300 VA (50Hz/60Hz)±1Hz

Fuse:

WARNING: Use specified fuse only. Fuse specification: 250V T3.15AH

EMC Description:

Do not use this device in close proximity to sources of strong electromagnetic radiation (e.g., unshielded intentional RF sources), as these may interfere with the proper operation. This equipment complies with the emission and immunity requirements of the EN61326–1:2006 and EN61326–2–6:2006.
NOTE: It is the manufacturer's responsibility to provide equipment electromagnetic compatibility information to the customer or user.
NOTE: It is the user's responsibility to ensure that a compatible electromagnetic environment for the equipment can be maintained in order that the device will perform as intended.

Sound:

Maximal sound: 65 dBA

Operating Environment:

Optimal operating temperature: 50°F - 86°F
Optimal operating humidity: 20% - 85%
Atmospheric pressure: 70 kPa - 106 kPa

Storage Environment:

Ambient temperature: -50°F - 104°F
Relative humidity: 10% - 90%
Atmospheric pressure: 50 kPa - 106 kPa

Running Environment:

Ambient temperature: 50°F - 104°F
Relative humidity: 10% - 90%
Atmospheric pressure: 70 kPa - 106 kPa
NOTE: Be sure to use and store the analyzer in the specified environment

Dimensions and Weight:

Element HT5 dimensions and weight

Element HT5 Control Assay Values

Control Lot BC1711B, exp 1/05/2018
Control Lot BC1709B, exp 11/05/2017

Element HT5 Maintenance and Control Log